AmScope B490

AmScope B490 Compound Binocular Microscope Instruction Manual

Модель: B490

1. Продукт закончилсяview

The AmScope B490 is a compound binocular microscope designed for biological and educational applications, offering high magnification and clear imaging. It features a sliding head for adjustable interpupillary distance, 10x widefield eyepieces, and a forward-facing nosepiece with four DIN achromatic objectives (4x, 10x, 40xS, 100xS oil). Illumination is provided by Brightfield halogen light with a rheostat for intensity control, paired with a 1.25 NA Abbe condenser and iris diaphragm. The microscope includes a double-layer mechanical stagе с какtage stop and graduated coarse and fine focus with tension control.

AmScope B490 Compound Binocular Microscope

Figure 1: AmScope B490 Compound Binocular Microscope, front-side view.

Включенные компоненты:

  • AmScope B490 microscope with double-layer mechanical stage
  • 10x widefield eyepieces (one pair)
  • 4x DIN achromatic objective
  • 10x DIN achromatic objective
  • 40xS DIN achromatic objective
  • 100xS (oil) DIN achromatic objective
  • Immersion oil (one bottle)
  • Color filters (blue and green)
  • Spare halogen bulb
  • Запасной предохранитель
  • Инструкция по эксплуатации
AmScope B490 Microscope Accessories

Figure 2: Included accessories: eyepieces, objectives, immersion oil, color filters, spare bulb, and fuse.

2. Инструкции по настройке

  1. Распаковка: Аккуратно извлеките все компоненты из упаковки. Сохраните оригинальную упаковку для дальнейшей транспортировки или хранения.
  2. Размещение: Place the microscope on a stable, level surface away from direct sunlight, excessive heat, dust, and vibrations.
  3. Головное собрание: Loosen the head retaining screw on the microscope body. Gently insert the binocular head into the top opening, ensuring it is fully seated. Tighten the retaining screw to secure the head.
  4. Установка окуляра: Remove the protective caps from the eyepiece tubes and the eyepieces. Insert the two WF10x eyepieces into the binocular head's eyepiece tubes.
  5. Объективная установка: Rotate the revolving nosepiece until the 4x objective is positioned over the stage opening. Install the remaining objectives (10x, 40xS, 100xS oil) clockwise into the nosepiece.
  6. Подключение питания: Ensure the power switch is in the 'OFF' position. Connect the power cord to the microscope's power input and then to a suitable electrical outlet (110V).
AmScope B490 Microscope Side View

Рисунок 3: Сторона view of the microscope, showing focus knobs and power input location.

3. Инструкция по эксплуатации

3.1 Электроэнергия и освещение

  1. Turn on the main power switch located on the base of the microscope.
  2. Adjust the illumination intensity using the rheostat knob on the side of the base. Start with a lower intensity and increase as needed for comfortable viewинж.

3.2 Размещение образца

  1. Rotate the revolving nosepiece to select the lowest power objective (4x).
  2. Поднимите сtage using the coarse focus knob to create enough space to place the specimen slide.
  3. Откройте сtage clips on the mechanical stage and carefully place the specimen slide, securing it with the clips.
  4. Используйте XYtage control knobs to position the desired area of the specimen directly under the objective lens.
AmScope B490 Microscope Front View

Рисунок 4: Передняя часть view of the microscope, highlighting the mechanical stage and objectives.

3.3 Фокусировка и увеличение

  1. Межзрачковое расстояние: While looking through the eyepieces, adjust the sliding head to match your eye distance until a single, circular field of view is observed. The range is 55 to 75mm.
  2. Диоптрийная регулировка: If one eye is stronger than the other, use the diopter adjustment ring on one eyepiece to compensate, ensuring both eyes are in focus.
  3. Грубая фокусировка: Slowly lower the stage using the coarse focus knob until the specimen comes into rough focus.
  4. Точная фокусировка: Используйте ручку точной фокусировки для точной фокусировки и получения четкого изображения.
  5. Изменение целей: To increase magnification, rotate the revolving nosepiece clockwise to the next objective (e.g., from 4x to 10x). Due to the parfocal design, only minor fine focus adjustments should be needed.
  6. Using 100xS Oil Immersion Objective: When using the 100xS objective, apply a small drop of immersion oil directly onto the specimen slide in the area to be viewed. Carefully lower the 100xS objective into the oil drop until it makes contact. Focus using the fine focus knob only. Always clean the 100xS objective and slide immediately after use to prevent oil from drying.
AmScope B490 Objectives and Stage Close-up

Рисунок 5: Крупный план view of the objectives and mechanical stage.

3.4 Condenser and Iris Diaphragm Adjustment

  1. The 1.25 NA Abbe condenser is mounted on a rack-and-pinion system, allowing its height to be adjusted. Raise or lower the condenser to optimize illumination for different objectives.
  2. The iris diaphragm lever controls the aperture of the light beam. Adjust it to achieve optimal contrast and resolution for the specimen and objective in use. Generally, a smaller aperture increases contrast but reduces resolution, while a larger aperture increases resolution but reduces contrast.

4. Техническое обслуживание

4.1 Очистка

  • Оптические компоненты: Use only specialized lens cleaning paper and lens cleaning solution. Gently wipe lenses in a circular motion. Avoid touching optical surfaces with bare hands.
  • Тело: Clean the microscope body with a soft, damp ткань. Избегайте использования агрессивных химикатов или растворителей.
  • Иммерсионное масло: After using the 100xS oil immersion objective, immediately clean the objective lens and the specimen slide with lens cleaning solution to prevent oil residue from hardening.

4.2 Замена лампочек

If the halogen illumination fails, the bulb may need replacement. Ensure the microscope is unplugged and the bulb has cooled before attempting replacement. Refer to the diagram in the original packaging for specific bulb access. Use the provided spare halogen bulb (6V/20W).

4.3 Замена предохранителя

If the microscope does not power on, check the fuse. Ensure the microscope is unplugged. The fuse holder is typically located near the power input. Replace with the provided spare fuse of the correct rating.

4.4 Хранение

When not in use, cover the microscope with a dust cover to protect it from dust and debris. Store in a cool, dry place. The anti-mold surfaces help preserve optics in high-humidity environments, but proper storage is still recommended.

5. Поиск Неисправностей

ПроблемаВозможная причинаРешение
Нет освещенияPower cord disconnected, power switch off, bulb burnt out, fuse blownCheck power connection, turn on switch, replace bulb, replace fuse
Изображение размытое/не в фокусеIncorrect focus, objective not fully engaged, dirty lenses, incorrect interpupillary distance/diopter settingAdjust coarse/fine focus, rotate nosepiece until objective clicks into place, clean lenses, adjust interpupillary distance/diopter
Insufficient contrastIris diaphragm too open, condenser height incorrectAdjust iris diaphragm to a smaller aperture, adjust condenser height
Темные пятна на поле зрения viewDust on eyepieces or objectives, dirty specimen slideClean eyepieces, objectives, and specimen slide
Stage not moving smoothlyStage lock engaged, debris in mechanismУбедитесь, чтоtage lock is disengaged, clean stage area (consult professional if persistent)

6. Технические характеристики

ОсобенностьСпецификация
ГоловаCompound Binocular, Sliding Head, 30-degree inclination, 360-degree rotation
ОкулярыWF10x
Цели4x, 10x, 40xS (spring), 100xS (spring, oil) DIN Achromatic
Диапазон увеличения40X-1000X
StageДвухслойная механическая Stage (140 x 140mm), 75 x 45mm traveling range, 1mm divisions, Stage Stop
ФокусировкаCoaxial Coarse and Fine Focus, 30mm focusing range, 0.002mm fine-focus divisions, Tension Control
ОсвещениеTransmitted (lower) Brightfield Halogen with Rheostat (6V/20W)
Конденсатор1.25 NA Abbe Condenser with Iris Diaphragm, Rack-and-Pinion Control
Расстояние между зрачками55-75мм
Власть110 В (американский стандарт)
Размеры (В x Ш x Г)16 x 9 x 12 дюйма (приблизительно)
Масса15.32 фунта (приблизительно)
МатериалSolid-metal enamel-coated body, optical glass components
Особые возможностиAnti-mold surfaces

7. Гарантия и поддержка

For warranty information, technical support, or service inquiries, please refer to the official AmScope website or contact AmScope customer service directly. Details regarding specific warranty periods and support procedures are typically provided with your purchase documentation or on the manufacturer's official channels.

Manufacturer: AmScope

Headquarters: Irvine, CA, USA

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